affi prep protein a resin (Bio-Rad)
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affi prep protein a resin
Affi Prep Protein A Resin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/affi+prep+protein+a+resin/Affi-Prep+Protein+A+Support/bio_rxiv__2025__08__20__670930-295-4-8
Average 93 stars, based on 278 article reviews
Affi Prep Protein A Resin, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/affi+prep+protein+a+resin/Affi-Prep+Protein+A+Support/bio_rxiv__2025__08__20__670930-295-4-8
Average 93 stars, based on 278 article reviews
affi prep protein a resin - by Bioz Stars,
2026-09
93/100 stars
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Incubation:Article Title: Characterization of membrane structures regulating primary ciliogenesis by quantitative isotropic ultrastructure imaging Article Snippet: .. Subsequently, 20 μl of Article Title: Paradoxical mitotic exit induced by a small molecule inhibitor of APC/CCdc20 Article Snippet: Cells for immunoprecipitation were lysed with HEPES buffer (20 mM HEPES pH 7.6 with KOH, 5 mM KCl, 1 mM DTT, 10 mM NaF, 1 mM Na3VO4 and Pierce protease inhibitor tablet) with apcin (50 μM) or DMSO for 30 min on ice and clarified by centrifuging at 20,000g for 10 min. .. Protein complexes were immunoprecipitated from 0.5–1 mg of total protein for 1 h at 4 °C with anti-Cdc27 (AF3.1) or anti-BubR1 antibody covalently coupled to Article Title: Paradoxical mitotic exit induced by a small molecule inhibitor of APC/CCdc20 Article Snippet: In brief, asynchronous HCT116 Cdc20WT and Cdc20D177A cells were collected and lysed with HEPES buffer (20 mM HEPES pH 7.6 with KOH, 5 mM KCl, 1 mM DTT, 10 mM NaF, 1 mM Na3VO4 and Pierce protease inhibitor tablet) for 30 min on ice and clarified by centrifugation at 20,000g for 10 min. .. Protein complexes were immunoprecipitated from ~3 mg of total protein for 1 h at 4 °C with anti-Cdc20 (H7) antibody covalently coupled to Article Title: Paradoxical mitotic exit induced by a small molecule inhibitor of APC/C Cdc20 Article Snippet: In brief, asynchronous HCT116 Cdc20 WT and Cdc20 D177A cells were collected and lysed with HEPES buffer (20 mM HEPES pH 7.6 w/ KOH, 5 mM KCl, 1 mM DTT, 10 mM NaF, 1 mM Na 3 VO 4 , and Pierce protease inhibitor tablet) for 30 minutes on ice and clarified by a 20,000x g spin for 10 minutes. .. Protein complexes were immunoprecipitated from ~3 mg of total protein for 1 hour at 4 °C with anti-Cdc20 (H7) antibody covalently coupled to Lysis:Article Title: Characterization of membrane structures regulating primary ciliogenesis by quantitative isotropic ultrastructure imaging Article Snippet: .. Subsequently, 20 μl of Article Title: 14-3-3 and erlin proteins differentially interact with RIPK2 complexes. Article Snippet: For immunoprecipitation of endogenous erlin-2, equal amounts of cell lysates were incubated with 2 μg specific antibodies (Sigma; HPA002025) at 4°C overnight. .. Immune complexes were collected with SDS Page:Article Title: Characterization of membrane structures regulating primary ciliogenesis by quantitative isotropic ultrastructure imaging Article Snippet: .. Subsequently, 20 μl of Immunoprecipitation:Article Title: Paradoxical mitotic exit induced by a small molecule inhibitor of APC/CCdc20 Article Snippet: Cells for immunoprecipitation were lysed with HEPES buffer (20 mM HEPES pH 7.6 with KOH, 5 mM KCl, 1 mM DTT, 10 mM NaF, 1 mM Na3VO4 and Pierce protease inhibitor tablet) with apcin (50 μM) or DMSO for 30 min on ice and clarified by centrifuging at 20,000g for 10 min. .. Protein complexes were immunoprecipitated from 0.5–1 mg of total protein for 1 h at 4 °C with anti-Cdc27 (AF3.1) or anti-BubR1 antibody covalently coupled to Article Title: Paradoxical mitotic exit induced by a small molecule inhibitor of APC/CCdc20 Article Snippet: In brief, asynchronous HCT116 Cdc20WT and Cdc20D177A cells were collected and lysed with HEPES buffer (20 mM HEPES pH 7.6 with KOH, 5 mM KCl, 1 mM DTT, 10 mM NaF, 1 mM Na3VO4 and Pierce protease inhibitor tablet) for 30 min on ice and clarified by centrifugation at 20,000g for 10 min. .. Protein complexes were immunoprecipitated from ~3 mg of total protein for 1 h at 4 °C with anti-Cdc20 (H7) antibody covalently coupled to Article Title: Paradoxical mitotic exit induced by a small molecule inhibitor of APC/C Cdc20 Article Snippet: In brief, asynchronous HCT116 Cdc20 WT and Cdc20 D177A cells were collected and lysed with HEPES buffer (20 mM HEPES pH 7.6 w/ KOH, 5 mM KCl, 1 mM DTT, 10 mM NaF, 1 mM Na 3 VO 4 , and Pierce protease inhibitor tablet) for 30 minutes on ice and clarified by a 20,000x g spin for 10 minutes. .. Protein complexes were immunoprecipitated from ~3 mg of total protein for 1 hour at 4 °C with anti-Cdc20 (H7) antibody covalently coupled to Chromatin Immunoprecipitation:Article Title: Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcriptional control Article Snippet: .. To prepare the beads, 20 mg of the different antibodies were loaded on 50 μL other:Article Title: Paradoxical mitotic exit induced by a small molecule inhibitor of APC/C Cdc20 Article Snippet: Cells for immunoprecipitation were lysed with HEPES buffer (20 mM HEPES pH 7.6 w/ KOH, 5 mM KCl, 1 mM DTT, 10 mM NaF, 1mM Na 3 VO 4 , and Pierce protease inhibitor tablet) with apcin (50 μM) or DMSO for 30 minutes on ice and clarified by a 20,000x g spin for 10 minutes. |
